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Transcriptomics and Selection Pressure Analysis Reveals the Influence Mechanism of PLIN1 Protein on the Development of Small Size in Min Pigs.

Qiao LiuLiqun YuZiwen ZhangYang ChangZhong-Hua LiuChunzhu Xu
Published in: International journal of molecular sciences (2023)
Body size is an important biological phenotypic trait that has attracted substantial attention. Small domestic pigs can serve as excellent animal models for biomedicine and also help meet sacrificial culture needs in human societies. Although the mechanisms underlying vertebral development regulating body size variation in domestic pigs during the embryonic period have been well described, few studies have examined the genetic basis of body size variation in post embryonic developmental stages. In this study, seven candidate genes- PLIN1 , LIPE , PNPLA1 , SCD , FABP5 , KRT10 and IVL -significantly associated with body size were identified in Min pigs, on the basis of weighted gene co-expression network analysis (WGCNA), and most of their functions were found to be associated with lipid deposition. Six candidate genes except for IVL were found to have been subjected to purifying selection. PLIN1 had the lowest ω value (0.139) and showed heterogeneous selective pressure among domestic pig lineages with different body sizes ( p < 0.05). These results suggested that PLIN1 is an important genetic factor regulating lipid deposition and consequently affecting body size variation in pigs. The culture of whole pig sacrifice in Manchu during the Qing Dynasty in China might have contributed to the strong artificial domestication and selection of Hebao pigs.
Keyphrases
  • network analysis
  • genome wide
  • poor prognosis
  • endothelial cells
  • copy number
  • magnetic resonance imaging
  • working memory
  • dna methylation
  • small molecule
  • transcription factor
  • long non coding rna
  • case control