Genome Analysis of Streptococcus pyogenes Associated with Pharyngitis and Skin Infections.
Joe IbrahimJonathan A EisenGuillaume JospinDavid A CoilGeorges KhazenSima TokajianPublished in: PloS one (2016)
Streptococcus pyogenes is a very important human pathogen, commonly associated with skin or throat infections but can also cause life-threatening situations including sepsis, streptococcal toxic shock syndrome, and necrotizing fasciitis. Various studies involving typing and molecular characterization of S. pyogenes have been published to date; however next-generation sequencing (NGS) studies provide a comprehensive collection of an organism's genetic variation. In this study, the genomes of nine S. pyogenes isolates associated with pharyngitis and skin infection were sequenced and studied for the presence of virulence genes, resistance elements, prophages, genomic recombination, and other genomic features. Additionally, a comparative phylogenetic analysis of the isolates with global clones highlighted their possible evolutionary lineage and their site of infection. The genomes were found to also house a multitude of features including gene regulation systems, virulence factors and antimicrobial resistance mechanisms.
Keyphrases
- antimicrobial resistance
- biofilm formation
- candida albicans
- soft tissue
- copy number
- genome wide
- wound healing
- genetic diversity
- pseudomonas aeruginosa
- escherichia coli
- staphylococcus aureus
- endothelial cells
- intensive care unit
- dna damage
- acute kidney injury
- systematic review
- dna methylation
- dna repair
- randomized controlled trial
- gene expression
- cystic fibrosis
- single cell
- case report
- septic shock
- transcription factor