Polymer-Based Terbium Complex as a Fluorescent Probe for Cancer Antigen 125 Detection: A Promising Tool for Early Diagnosis of Ovarian Cancer.
Magda M MohamedHisham GamalAkram El-DidamonyAhmed O YoussefEsraa ElshahatEkram H MohamedMohamed S AttiaPublished in: ACS omega (2024)
A novel photoprobe, Tb-acetylacetone (Tb-ACAC) doped within a modified epoxy cellulose polymer immobilized with CA-125 monoclonal antibody, offers an accurate and highly selective method for early ovarian cancer (OC) diagnosis by detecting cancer antigen 125 (CA-125) in serum samples. This approach leverages quenching of the Tb-ACAC luminescence upon binding to CA-125. Characterization of the photoprobe film through UV-vis and fluorescence measurements confirmed the presence of Tb-ACAC within the polymer matrix. In aqueous solution (pH 6.8, λ ex = 365 nm), the characteristic emission band of Tb-ACAC at λ em = 546.2 nm exhibited significant quenching upon CA-125 binding. This quenching effect enabled the sensitive and specific detection of CA-125 in diverse serum samples from OC patients, demonstrating the applicability, simplicity, and effectiveness of this novel approach.
Keyphrases
- mycobacterium tuberculosis
- aqueous solution
- energy transfer
- fluorescent probe
- monoclonal antibody
- papillary thyroid
- end stage renal disease
- protein kinase
- quantum dots
- living cells
- ejection fraction
- randomized controlled trial
- chronic kidney disease
- newly diagnosed
- photodynamic therapy
- ionic liquid
- high resolution
- peritoneal dialysis
- squamous cell carcinoma
- room temperature
- real time pcr
- label free
- systematic review
- loop mediated isothermal amplification
- single molecule
- reduced graphene oxide
- childhood cancer
- highly efficient
- transcription factor
- solid state
- patient reported
- magnetic nanoparticles
- dna binding