Anti-Inflammatory Activity of Marine Ovothiol A in an In Vitro Model of Endothelial Dysfunction Induced by Hyperglycemia.
Immacolata CastellanoPamela Di TomoNatalia Di PietroDomitilla MandatoriCaterina PipinoGloria FormosoAlessandra NapolitanoAnna PalumboAssunta PandolfiPublished in: Oxidative medicine and cellular longevity (2018)
Chronic hyperglycemia is associated with oxidative stress and vascular inflammation, both leading to endothelial dysfunction and cardiovascular disease that can be weakened by antioxidant/anti-inflammatory molecules in both healthy and diabetic subjects. Among natural molecules, ovothiol A, produced in sea urchin eggs to protect eggs/embryos from the oxidative burst at fertilization and during development, has been receiving increasing interest for its use as an antioxidant. Here, we evaluated the potential antioxidative/anti-inflammatory effect of purified ovothiol A in an in vitro cellular model of hyperglycemia-induced endothelial dysfunction employing human umbilical vein endothelial cells (HUVECs) from women affected by gestational diabetes (GD) and from healthy mothers. Ovothiol A was rapidly taken up by both cellular systems, resulting in increased glutathione values in GD-HUVECs, likely due to the formation of reduced ovothiol A. In tumor necrosis factor-α-stimulated cells, ovothiol A induced a downregulation of adhesion molecule expression and decrease in monocyte-HUVEC interaction. This was associated with a reduction in reactive oxygen and nitrogen species and an increase in nitric oxide bioavailability. These results point to the potential antiatherogenic properties of the natural antioxidant ovothiol A and support its therapeutic potential in pathologies related to cardiovascular diseases associated with oxidative/inflammatory stress and endothelial dysfunction.
Keyphrases
- oxidative stress
- diabetic rats
- anti inflammatory
- induced apoptosis
- cardiovascular disease
- endothelial cells
- high glucose
- nitric oxide
- ischemia reperfusion injury
- dna damage
- type diabetes
- rheumatoid arthritis
- signaling pathway
- cell proliferation
- poor prognosis
- cell cycle arrest
- binding protein
- metabolic syndrome
- pregnancy outcomes
- cardiovascular risk factors
- staphylococcus aureus
- adipose tissue
- cardiovascular events
- escherichia coli
- pseudomonas aeruginosa
- wound healing
- coronary artery disease
- candida albicans
- insulin resistance