Phenolic and Steroidal Metabolites from the Cultivated Edible Inonotus hispidus Mushroom and Their Bioactivities.
Rong-Wei KouShuang-Tian DuBing XiaQiang ZhangXia YinJin-Ming GaoPublished in: Journal of agricultural and food chemistry (2021)
A chemical study on the fruiting bodies of cultivated edible mushroom Inonotus hispidus resulted in 14 metabolites including three new hispolon congeners, named inonophenols A-B and one new lanostane triterpenoid, named inonoterpene A. These structures were identified by NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) data analysis. All metabolites were assessed for neurotrophic, anti-inflammatory, and antioxidative activities. Among them, inonophenols B and C were the most active in promoting PC-12 cell neurite outgrowth at a concentration of 10 μM. The phenolic derivatives reduced NO generation by lipopolysaccharide (LPS)-induced BV-2 microglial cells by suppressing the expression of toll-like receptor-4 (TLR-4) and the nuclear factor-kappa-B (NF-κB) signaling pathway as well as the inflammatory mediators including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, the phenolics showed antioxidant effects in DPPH scavenging assay with the IC50 values of 9.82-21.43 μM. These findings showed that I. hispidus may be a new source of neurotrophic and protective agents against neurodegenerative disorders.
Keyphrases
- toll like receptor
- lps induced
- nuclear factor
- inflammatory response
- nitric oxide synthase
- high resolution
- anti inflammatory
- data analysis
- nitric oxide
- ms ms
- mass spectrometry
- lipopolysaccharide induced
- induced apoptosis
- signaling pathway
- oxidative stress
- poor prognosis
- cell cycle arrest
- single cell
- immune response
- pi k akt
- high throughput
- magnetic resonance
- endoplasmic reticulum stress
- liquid chromatography
- stem cells
- anti inflammatory drugs
- epithelial mesenchymal transition
- cell therapy
- capillary electrophoresis
- high speed
- high performance liquid chromatography
- binding protein
- cell death
- spinal cord injury
- long non coding rna
- cell proliferation