A Novel Synthetic, Xeno-Free Biomimetic Surface for Serum-Free Expansion of Human Mesenchymal Stromal Cells.
Kristina ThammKristin MöbusRussell TowersSandra SegeletzRichard WetzelMartin BornhäuserYixin ZhangManja WobusPublished in: Advanced biosystems (2020)
Human mesenchymal stromal cells (hMSCs) have enormous potential for the treatment of various inflammatory and degenerative diseases. Their manufacturing for cell-based therapies requires extensive ex vivo expansion and optimal growth conditions. To support cell adhesion, spreading, and growth in serum-free culture conditions, the applied plasticware needs to be functionalized with essential biochemical cues. By employing a recently developed screening tool, a chemically defined functional matrix composed of dextran sulfate and a bone-related extracellular matrix peptide is identified, which supports long-term culture of bone marrow-derived hMSCs in serum-free culture conditions. Cells grown under these conditions display rapid proliferation and high viability while maintaining their differentiation and immunomodulatory capacity, characteristic cell morphology, expression of hMSC-specific surface antigens as well as important markers of stemness and differentiation potential. The chemically defined, serum-free culture environment enables reliable and reproducible expansion of hMSCs important for cell based-therapies, drug screening, and disease modeling.
Keyphrases
- extracellular matrix
- single cell
- endothelial cells
- cell therapy
- cell adhesion
- bone marrow
- stem cells
- mesenchymal stem cells
- poor prognosis
- induced apoptosis
- signaling pathway
- induced pluripotent stem cells
- risk assessment
- cell cycle arrest
- dendritic cells
- quantum dots
- cell death
- bone mineral density
- human health
- immune response
- endoplasmic reticulum stress
- adverse drug
- binding protein
- replacement therapy