Microaspiration of Solanum tuberosum root cells at early stages of infection by Globodera pallida.

Rinu KooliyottilLouise-Marie DandurandJoseph C KuhlAllan CaplanFangming Xiao

Published in: Plant methods (2017)

The use of PKH26 stained nematode juveniles enabled early detection of nematode infected cells for microaspiration. To investigate transcriptional changes in low yielding RNA samples, bead-based RNA extraction procedures minimized RNA degradation and provided high quality RNA. This protocol provides a robust procedure to analyze gene expression in nematode-infected cells.

Keyphrases

induced apoptosis
gene expression
cell cycle arrest
randomized controlled trial
endoplasmic reticulum stress
oxidative stress
transcription factor
minimally invasive
cell proliferation
pi k akt