A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization.

Britta EggenreichVignesh RajamanickamDavid Johannes WurmJens FrickeChristoph HerwigOliver Spadiut

Published in: Microbial cell factories (2017)

A combination of HPLC and automated data analysis describes a valuable, novel tool to monitor and evaluate cell disruption processes. Our methodology, which can be used both in upstream (USP) and downstream processing (DSP), describes a valuable tool to evaluate cell disruption processes as it can be implemented at-line, gives results within minutes after sampling and does not need manual intervention.

Keyphrases

data analysis
ms ms
single cell
machine learning
simultaneous determination
cell therapy
deep learning
high throughput
randomized controlled trial
high performance liquid chromatography
solid phase extraction
mass spectrometry
bone marrow
mesenchymal stem cells