Cancer-Targeted Azo Dye for Two-Photon Photodynamic Therapy in Human Colon Tissue.
Dong Joon LeeVinayak JuvekarHyo Won LeeEun Seo KimChoong-Kyun NohSung Jae ShinHwan Myung KimPublished in: Analytical chemistry (2021)
Inappropriate cancer management can be prevented by simultaneous cancer diagnosis, treatment, and real-time assessment of therapeutic processes. Here, we describe the design of a two-photon (TP) photosensitizer (PS), ACC-B, for high temporal and spatioselective near-infrared cancer therapy. ACC-B consisting of a biotin unit significantly enhanced the cancer sensitivity of the PS. Upon TP irradiation, ACC-B generated reactive oxygen species (ROS) through the type I photodynamic therapy (PDT) process and triggered highly selective cancer ablation. In addition, fluorescence microscopy images revealed that ACC-B-loaded live human colon tissues showed a marked difference in ACC-B uptake between normal and cancer tissues, and this property was used for real-time imaging. Upon 770 nm TP treatment, ACC-B generated ROS efficiently in live colon cancer tissues with high spatial selectivity. During PDT, ACC-B can provide in situ spatioselective visualization of cellular behavior and molecular information for therapeutic assessment in specific regions.
Keyphrases
- photodynamic therapy
- papillary thyroid
- cancer therapy
- squamous cell
- reactive oxygen species
- endothelial cells
- gene expression
- drug delivery
- healthcare
- cell death
- lymph node metastasis
- optical coherence tomography
- single molecule
- childhood cancer
- fluorescence imaging
- radiation induced
- mass spectrometry
- machine learning
- smoking cessation
- single cell
- young adults
- atrial fibrillation
- pluripotent stem cells
- radiation therapy
- replacement therapy
- clinical evaluation