A New Ratiometric Lysosomal Copper(II) Fluorescent Probe To Map a Dynamic Metallome in Live Cells.

Maria Laura GiuffridaGiuseppe Trusso SfrazzettoCristina SatrianoStefania ZimboneGaetano A TomaselliAgata CopaniEnrico Rizzarelli

Published in: Inorganic chemistry (2018)

We synthesized a new ratiometric fluorescent Cu2+ probe, bearing a morpholine moiety for selective binding to lysosomes and two picolylamine arms for the specific chelation of divalent copper ions. The probe capability to detect lysosomal Cu2+ was demonstrated in human differentiated neuroblastoma cells by confocal microscopy.

Keyphrases

living cells
fluorescent probe
quantum dots
induced apoptosis
cell cycle arrest
single molecule
endothelial cells
sensitive detection
endoplasmic reticulum stress
oxidative stress
oxide nanoparticles
signaling pathway
cell death
nitric oxide
hydrogen peroxide
cell proliferation
high density
pluripotent stem cells