MALDI-TOF Mass Spectrometry Analysis and Human Post-Mortem Microbial Community: A Pilot Study.
Carlo Pietro CampobassoGennaro MastroianniAlessandro FeolaPasquale MascoloAnna CarforaBruno LiguoriPierluca ZanganiFederica Dell'AnnunziataVeronica FollieroArianna PetrilloMaria Elena Della PepaFrancesca MartoraMarilena GaldieroPublished in: International journal of environmental research and public health (2022)
MALDI-TOF is a promising method for PMI estimation, given its sample handling, good reproducibility, and high speed and throughput. Although several intrinsic and extrinsic factors can affect the structure of the HPM, MALDI-TOF can detect the overall microbial community turnover of most prevalent phyla during decomposition. Limitations are mainly related to its sensitivity due to the culture-dependent method and bias in the identification of new isolates.
Keyphrases
- mass spectrometry
- microbial community
- high speed
- liquid chromatography
- high resolution
- antibiotic resistance genes
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- atomic force microscopy
- endothelial cells
- bone mineral density
- induced pluripotent stem cells
- pluripotent stem cells
- tandem mass spectrometry
- genetic diversity
- body composition
- drug induced
- bioinformatics analysis
- wastewater treatment