A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice.

Xu TangGuanqing LiuJianping ZhouQiurong RenQi YouLi TianXuhui XinZhaohui ZhongBinglin LiuXuelian ZhengDengwei ZhangAimee MalzahnZhiyun GongYiping QiTao ZhangYong Zhang

Published in: Genome biology (2018)

Our comprehensive and rigorous analysis of WGS data across multiple sample types suggests both Cas9 and Cpf1 nucleases are very specific in generating targeted DNA modifications and off-targeting can be avoided by designing guide RNAs with high specificity.

Keyphrases

genome editing
crispr cas
cancer therapy
electronic health record
single molecule
big data
cell free
machine learning
artificial intelligence