Optimized CUBIC protocol for three-dimensional imaging of chicken embryos at single-cell resolution.

María Victoria Gómez-GaviroEvan BalabanDiana BocanceaMaría Teresa LorrioMaria PompeianoManuel Desco Jorge Ripoll Juan José Vaquero

Published in: Development (Cambridge, England) (2017)

The CUBIC tissue-clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole brains of late-stage embryos.

Keyphrases

single cell
high resolution
early stage
randomized controlled trial
single molecule
rna seq
high throughput
optical coherence tomography
fluorescence imaging
high speed
machine learning
pregnant women
radiation therapy
label free
loop mediated isothermal amplification