CD20 Expression as a Possible Novel Prognostic Marker in CLL: Application of EuroFlow Standardization Technique and Normalization Procedures in Flow Cytometric Expression Analysis.
Anke SchilhabelPeter Jonas WalterPaula CramerJulia von TresckowSaskia KohlscheenMonika SzczepanowskiAnna LaquaKirsten FischerBarbara EichhorstSebastian BöttcherChristof SchneiderEugen TauschMonika BrüggemannMichael KnebaMichael HallekMatthias RitgenPublished in: Cancers (2022)
Standardized staining and instrument monitoring enable a robust assessment of longitudinal biological variations of marker expression based on MFI values. Obinutuzumab showed a higher proportion of patients with a strong MRD response independent from initial CD20 expression, whereas high pre-therapeutic CD20 expression levels seem to correlate with a profound response to Ofatumumab.