Sensitivity optimisation of tuberculosis bioaerosol sampling.
Benjamin PattersonRyan DinkeleSophia GessnerCarl MorrowMireille KamarizaCarolyn R BertozziAndrew KamholzWayne BrydenCharles CallDigby F WarnerRobin WoodPublished in: PloS one (2020)
This study demonstrates that technical improvements in particle collection together with sensitive detection enable rapid quantitation of viable Mtb in bioaerosols of sputum positive TB cases. Increased sampling sensitivity may allow future TB transmission studies to be extended to sputum-negative and subclinical individuals, and suggests the potential utility of bioaerosol measurement for rapid intervention in other airborne infectious diseases.
Keyphrases
- mycobacterium tuberculosis
- sensitive detection
- pulmonary tuberculosis
- loop mediated isothermal amplification
- infectious diseases
- randomized controlled trial
- quantum dots
- ms ms
- mass spectrometry
- particulate matter
- current status
- emergency department
- liquid chromatography
- risk assessment
- hepatitis c virus
- tandem mass spectrometry
- electronic health record