Aberrant methylation of miR-124 upregulates DNMT3B in colorectal cancer to accelerate invasion and migration.
Shiva ShahmohamadnejadZahra Nouri GhonbalaniBehnoosh TahbazlahafiGhodratollah PanahiReza MeshkaniAmirnader Emami RazaviHajar Shokri AfraEhsan KhaliliPublished in: Archives of physiology and biochemistry (2020)
The dysregulation of microRNA expression is significantly associated with the initiation and development of CRC. miR-124 is markedly downregulated in colorectal cancer. In the present study, the effects of methylation, over expression and downregulation of miR-124 and its target gene DNMT3B on the proliferation, migration and invasion of colorectal cell line were investigated. The promoter methylation status of miR-124 in the CRC was investigated by methylation specific PCR (MSP). The potential role of miR-124 expression in CRC cells was investigated using the demethylation reagent 5-Aza-CdR and transfection of miR-124 mimic/antimir. MSP revealed that miR-124 promoter region was hypermethylated, result in its significant downregulation in tumour tissues. We showed miR-124 expression was upregulated following 5-AZA-CdR treatment. Transfected Hct-116 cell line with miR-124 leads to decreased DNMT3B expression, cell proliferation, migration and invasion of HCT-116. In conclusion, our data indicate that miR-124 suppress colorectal cancer proliferation, migration and invasion through downregulating DNMT3B level.
Keyphrases
- cell proliferation
- long non coding rna
- poor prognosis
- dna methylation
- long noncoding rna
- cell cycle
- genome wide
- gene expression
- pi k akt
- signaling pathway
- cell death
- binding protein
- transcription factor
- high resolution
- single cell
- cell cycle arrest
- mass spectrometry
- deep learning
- risk assessment
- electronic health record
- combination therapy
- artificial intelligence
- smoking cessation