Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy.
Elena KirilovaIlona MickevicaLigita MezaraupeAleksandrs PuckinsIlze RubeninaSergejs OsipovsInese KokinaAndrejs BulanovsMuza KirjusinaInese GavaranePublished in: Luminescence : the journal of biological and chemical luminescence (2019)
In the present study a new luminescent dye 3-N-(2-pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3-aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.
Keyphrases
- single molecule
- high resolution
- optical coherence tomography
- energy transfer
- label free
- electron microscopy
- highly efficient
- high speed
- living cells
- quantum dots
- randomized controlled trial
- high throughput
- aqueous solution
- molecular docking
- raman spectroscopy
- pregnancy outcomes
- oxidative stress
- visible light
- ionic liquid
- cell death
- molecular dynamics simulations
- fluorescent probe