Adipogenesis of ear mesenchymal stem cells (EMSCs): adipose biomarker-based assessment of genetic variation, adipocyte function, and brown/brite differentiation.
Dinh-Toi ChuDang Tien TruongHue Vu ThiNguyen Thi Lan HuongPublished in: Molecular and cellular biochemistry (2022)
Ear mesenchymal stem cells (EMSCs) have been investigated to differentiate into adipocytes, chondrocytes, and muscle cells in vitro. However, the factors controlling adipogenesis of this stem cell population in vitro, function, and type of adipocytes raised from them are still unclear. Here we found that genetics have a modest effect on adipogenic capacity of EMSCs. Adipocytes differentiated from EMSCs have a potential function in lipid metabolism as indicated by expression of lipogenic genes and this function of EMSC adipocytes is regulated by genetics. EMSCs failed to be differentiated into brite/brown adipocytes due to their lack of a thermogenic program, but adipocytes raised from EMSCs showed a fate of white adipocytes. Overall, our data suggest that EMSCs differentiate into functional white adipocytes in vitro and this is genetic-dependent.
Keyphrases
- adipose tissue
- high fat diet induced
- mesenchymal stem cells
- insulin resistance
- stem cells
- poor prognosis
- genome wide
- bone marrow
- metabolic syndrome
- induced apoptosis
- oxidative stress
- risk assessment
- electronic health record
- gene expression
- copy number
- signaling pathway
- mass spectrometry
- binding protein
- cell cycle arrest
- cell therapy
- data analysis
- high speed