A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.

Dae-Kyum KimJennifer J KnappDa KuangAditya ChawlaPatricia CassonnetHunsang LeeDayag SheykhkarimliPayman Samavarchi-TehraniHala AbdouniAshyad RayhanRoujia LiOxana PogoutseÉtienne CoyaudSylvie van der WerfCaroline DemeretAnne-Claude GingrasMikko TaipaleBrian RaughtYves JacobFrederick P Roth

Published in: G3 (Bethesda, Md.) (2020)

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Keyphrases

sars cov
respiratory syndrome coronavirus
life cycle
poor prognosis
binding protein
single molecule
electron transfer