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Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigs.

Ohnmar MyintAyako YoshidaSatoshi SekiguchiNguyen Van DiepNaoyuki FukeUda Zahli IzzatiTakuya HiraiRyoji Yamaguchi
Published in: BMC veterinary research (2019)
The developed indirect ELISA test in our study would be useful as a reliable test for serological survey and disease control of PEDV infection, and our pre-antigen coated ELISA plates can be preserved at 4 °C until at least 6 months.
Keyphrases
  • high throughput
  • cross sectional
  • monoclonal antibody
  • label free
  • loop mediated isothermal amplification
  • real time pcr