Improving CRISPR-Cas9 directed faithful transgene integration outcomes by reducing unwanted random DNA integration.

Rio HermantaraLaura RichmondAqeel Faisal TaqiSabari ChilakaValentine JeantetIleana GuerriniKatherine WestAdam West

Published in: Journal of biomedical science (2024)

Our results highlight the need for a more stringent assessment of CRISPR-Cas9-mediated knock-in outcomes, and the importance of careful strategy design to maximise efficient and faithful transgene integration.

Keyphrases

crispr cas
genome editing
circulating tumor
single molecule
type diabetes
cell free
skeletal muscle
glycemic control