Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples.
Marina ArenasJulia MartínJuan Luis SantosIrene AparicioOmar Fernández-SanfranciscoEsteban AlonsoPublished in: Molecules (Basel, Switzerland) (2022)
Platinum-based cytostatic drugs are one of the most widely used cancer treatments. They are excreted via the urinary tract and can reach the environment through wastewater, posing a risk to human health due to their side effects. Four identification and quantification techniques, including liquid chromatography (LC) separation coupled to (i) a diode array ultraviolet (UV(DAD)) (ii), mass spectrometer in single ion monitoring mode (LC-MS) and (iii) multiple reaction monitoring mode (LC-MS/MS) and (iv) derivatization with diethyldithiocarbamate prior to LC-MS/MS analysis, have been optimized and compared for the multiresidue determination of main platinized cytostatic drugs (cisplatin, carboplatin, and oxaliplatin) in urine samples. Parameters that affect the efficiency of the chromatographic separation and analytical determination of different methods (column, mobile phase, wavelength, precursor ions, fragmentor, and product ions) were optimized. Analytical features, such as matrix effect, sensitivity, precision, selectivity, and linearity, were calculated. In terms of selectivity, the derivatization technique was discarded since it was only applicable to the platinated sum. A high dilution of the sample with LC-UV(DAD) was needed to reduce the matrix effect. Overall, the LC-MS/MS method presented the best analytical features (% RSD ≤ 12.8%, R 2 ≥ 0.991, or method-detection limits between 0.01-1 µg mL -1 ). The selected method was applied to the quantification of platinized cytostatic drugs in hospital urine samples from oncologic patients.
Keyphrases
- liquid chromatography
- simultaneous determination
- solid phase extraction
- tandem mass spectrometry
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- high performance liquid chromatography
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- molecularly imprinted
- gas chromatography
- human health
- gas chromatography mass spectrometry
- urinary tract
- high resolution
- ms ms
- risk assessment
- quantum dots
- end stage renal disease
- ejection fraction
- prognostic factors
- healthcare
- papillary thyroid
- aqueous solution
- clinical trial
- prostate cancer
- wastewater treatment
- loop mediated isothermal amplification
- anaerobic digestion
- acute care
- structural basis
- peritoneal dialysis
- climate change
- radiation therapy
- randomized controlled trial