Oxysophocarpine protects airway epithelial cells against inflammation and apoptosis by inhibiting miR-155 expression.
Linfu LiRenbing ShiWeimei ShiRui ZhangLonghuo WuPublished in: Future medicinal chemistry (2020)
Oxysophocarpine (OSC) has been documented for anti-inflammatory activity. However, the mechanisms of OSC in anti-inflammation are unclear. Aim: To investigate the protective effects of OSC on inflammation and apoptosis induced by lipopolysaccharide in NCI-H292 and human primary airway epithelial cells. Materials & methods: MTT and Annexin V-FITC/PI staining were used to detect cells viability. Inflammatory responses were determined by ELISA. The quantitative real-time PCR (qRT-PCR) and western blot were used to detect mRNA/miRNA and protein expressions respectively. Co-immunoprecipitation was investigated for protein interactions. Results & conclusion: miR-155 mimics significantly induced cell apoptosis, inflammatory responses and MAPK and NF-κB pathways. NDFIP1 was identified as the target of miR-155. OSC protected cells against apoptosis and inflammatory responses and compromised miR-155 activity by attenuating MAPK and NF-κB pathways.
Keyphrases
- cell cycle arrest
- pi k akt
- oxidative stress
- cell proliferation
- induced apoptosis
- signaling pathway
- long non coding rna
- diabetic rats
- long noncoding rna
- endoplasmic reticulum stress
- cell death
- poor prognosis
- real time pcr
- binding protein
- endothelial cells
- lps induced
- toll like receptor
- south africa
- amino acid
- protein protein
- atomic force microscopy
- high speed