A Fast and Selective Approach for Profiling Vicinal Diols Using Liquid Chromatography-Post Column Derivatization-Double Precursor Ion Scanning Mass Spectrometry.
Debin WanChristophe MorisseauBruce D HammockJun YangPublished in: Molecules (Basel, Switzerland) (2022)
Vicinal diols are important signaling metabolites of various inflammatory diseases, and some of them are potential biomarkers for some diseases. Utilizing the rapid reaction between diol and 6-bromo-3-pyridinylboronic acid (BPBA), a selective and sensitive approach was established to profile these vicinal diols using liquid chromatography-post column derivatization coupled with double precursor ion scan-mass spectrometry (LC-PCD-DPIS-MS). After derivatization, all BPBA-vicinal-diol esters gave a pair of characteristic isotope ions resulting from 79 Br and 81 Br. The unique isotope pattern generated two characteristic fragment ions of m/z 200 and 202. Compared to a traditional offline derivatization technique, the new LC-PCD-DPIS-MS method retained the capacity of LC separation. In addition, it is more sensitive and selective than a full scan MS method. As an application, an in vitro study of the metabolism of epoxy fatty acids by human soluble epoxide hydrolase was tested. These vicinal-diol metabolites of individual regioisomers from different types of polyunsaturated fatty acids were easily identified. The limit of detection (LOD) reached as low as 25 nM. The newly developed LC-PCD-DPIS-MS method shows significant advantages in improving the selectivity and therefore can be employed as a powerful tool for profiling vicinal-diol compounds from biological matrices.
Keyphrases
- liquid chromatography
- mass spectrometry
- gas chromatography
- tandem mass spectrometry
- simultaneous determination
- high resolution mass spectrometry
- high performance liquid chromatography
- ultra high performance liquid chromatography
- ms ms
- solid phase extraction
- high resolution
- capillary electrophoresis
- liquid chromatography tandem mass spectrometry
- gas chromatography mass spectrometry
- computed tomography
- quantum dots
- fatty acid
- endothelial cells
- loop mediated isothermal amplification
- multiple sclerosis
- single cell
- magnetic resonance
- magnetic resonance imaging
- aqueous solution