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Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system.

Jun SunQingzhuo WangYu JiangZhiqiang WenLirong YangJianping WuSheng Yang
Published in: Microbial cell factories (2018)
In this research, we established CRISPR based genome editing and regulation control systems in P. putida KT2440. These fast and efficient approaches will greatly facilitate the application of P. putida KT2440.
Keyphrases
  • genome editing
  • crispr cas
  • gene expression
  • transcription factor
  • biofilm formation
  • genome wide
  • staphylococcus aureus
  • pseudomonas aeruginosa
  • dna methylation
  • cystic fibrosis