Ultrasmall Bi/Cu Coordination Polymer Combined with Glucose Oxidase for Tumor Enhanced Chemodynamic Therapy by Starvation & Photothermal Treatment.

Multi-modal combination therapy for tumor is expected to have superior therapeutic effect comparing with monotherapy. In this study, a super-small bismuth/copper-gallic acid coordination polymer nanoparticle (BCN) protected by polyvinylpyrrolidone (PVP) is designed, which is co-encapsulated with glucose oxidase (GOX) by phospholipid to obtain nanoprobe BCGN@L. It shows that the BCN particle has an average size of 1.8±0.7 nm, and photothermal conversion of BCGN@L is 31.35% for photothermal imaging and PTT. During the treatment process of 4T1 tumor-bearing nude mice, GOX catalyzes glucose in the tumor tissue to generate gluconic acid and hydrogen peroxide (H 2 O 2 ), which reacts with copper ions (Cu 2+ ) to produce toxic hydroxyl radicals (•OH) for chemodynamic therapy(CDT) and new fresh oxygen (O 2 ) to supply to GOX for further catalysis, preventing tumor hypoxia. These reactions increase glucose depletion for starvation therapy (ST), decrease heat shock protein expression, enhance tumor sensitivity to low-temperature PTT. The in vitro and in vivo results demonstrate that the combination of CDT with other treatments produces an excellent tumor growth inhibition. Blood biochemistry and histology analysis suggests that the nanoprobe has negligible toxicity. All the positive results reveal that the nanoprobe can be a promising approach for incorporation into multi-modal anticancer therapy. This article is protected by copyright. All rights reserved.