Transcriptional Reprogramming of Autographa Californica Multiple Nucleopolyhedrovirus Chitinase and Cathepsin Genes Enhances Virulence.
Jeffrey J HodgsonA Lorena PassarelliPeter J KrellPublished in: Viruses (2023)
The baculoviral chitinase (CHIA) and cathepsin (V-CATH) enzymes promote terminal insect host liquefaction, which aids viral progeny dissemination. Recombinant Autographa californica nucleopolyhedrovirus (AcMNPV)-derived viruses were previously generated with reprogrammed chiA transcription by replacing the native promoter with the AcMNPV polyhedrin ( polh ) or core protein ( p6.9 ) promoter sequences, but of both these chiA -reprogrammed viruses lacked v-cath transcription and V-CATH enzymatic activity. Here, we report that dual p6.9 / polh promoter reprogramming of the adjacent chiA/v-cath genes resulted in modulated temporal transcription of both genes without impacting infectious budded virus production. These promoter changes increased CHIA and V-CATH enzyme activities in infected Spodoptera frugiperda -derived cultured cells and Trichoplusia ni larvae. In addition, larvae infected with the dual reprogrammed virus had earlier mortalities and liquefaction. This recombinant baculovirus, lacking exogenous genomic elements and increased chiA/v-cath expression levels, may be desirable for and amenable to producing enhanced baculovirus-based biopesticides.
Keyphrases
- transcription factor
- dna methylation
- genome wide identification
- genome wide
- gene expression
- poor prognosis
- staphylococcus aureus
- bioinformatics analysis
- escherichia coli
- pseudomonas aeruginosa
- aedes aegypti
- induced apoptosis
- sars cov
- hydrogen peroxide
- copy number
- antimicrobial resistance
- sewage sludge
- long non coding rna
- antiretroviral therapy
- biofilm formation
- zika virus
- drosophila melanogaster
- risk assessment
- cell proliferation
- protein protein
- heavy metals
- cell death
- heat stress
- disease virus