Testing protocol affects the velocity at VO2max in semi-professional soccer players.

To compare three different protocols to assess the velocity associated with the maximal oxygen uptake (Vmax) in soccer players. Sixteen semi-professional soccer players performed three maximum incremental tests on treadmill: two continuous protocols [1 km·h-1·min-1 (CP1); and 1 km·h-1 every 2 min (CP2)], and one discontinuous (DP) protocol to determine Vmax, maximum oxygen uptake (VO2max) and oxygen cost of running (i.e., the slope of the VO2 vs velocity relationship at submaximal exercise). Vmax was higher in CP1> CP2> DP (19.4 ± 1.7, 17.4 ± 1.2, 16.1 ± 1.1 km·h-1 for CP1, CP2, and DP, respectively; P < 0.05 ES: 0.09 to 3.36). No difference in VO2max was found between CP1, CP2 and DP (P > 0.05). Oxygen cost of running showed between-protocol differences (CP1> CP2> DP; P < 0.05; ES: 0.28 to 3.30). Vmax was higher when determined using continuous vs discontinuous protocols due to the greater overestimation in oxygen cost of running. Such differences in Vmax should be considered to optimize acute physiological responses during high-intensity running activities.