CAR-T cells and TRUCKs that recognize an EBNA-3C-derived epitope presented on HLA-B*35 control Epstein-Barr virus-associated lymphoproliferation.
Anna Christina DragonKatharina ZimmermannThomas NerreterDeborah SandfortJulia LahrbergStephan KlößChristina KlothCaroline MangareAgnes BonifaciusSabine Tischer-ZimmermannRainer BlasczykBritta Maecker-KolhoffBarbara Uchanska-ZieglerHinrich AbkenAxel SchambachMichael HudecekBritta Eiz-VesperPublished in: Journal for immunotherapy of cancer (2021)
Our results demonstrate that TÜ165 CAR-Ts recognize EBV peptide/HLA complexes in a TCR-like manner and thereby allow for recognizing an intracellular EBV target. TÜ165 TRUCKs equipped with inducible IL-12 expression responded even more effectively and released IL-12 recruited additional immune cells which are generally missing in proximity of lymphoproliferation in immunocompromised PTLD patients. This suggests a new and promising strategy to specifically target EBV-infected cells while sparing and mobilizing healthy immune cells and thereby enable control of EBV-associated lymphoproliferation.
Keyphrases
- epstein barr virus
- diffuse large b cell lymphoma
- induced apoptosis
- cell cycle arrest
- end stage renal disease
- newly diagnosed
- poor prognosis
- chronic kidney disease
- prognostic factors
- intensive care unit
- cell death
- peritoneal dialysis
- immune response
- reactive oxygen species
- monoclonal antibody
- acute respiratory distress syndrome
- patient reported