High-Precision Single-Cell microRNA Therapy by a Functional Nanopipette with Sensitive Photoelectrochemical Feedback.
You-Wei ZhengSi-Yuan YuZheng LiYi-Tong XuWei-Wei ZhaoDechen JiangHong-Yuan ChenJing-Juan XuPublished in: Small (Weinheim an der Bergstrasse, Germany) (2023)
This work proposes the concept of single-cell microRNA (miR) therapy and proof-of-concept by engineering a nanopipette for high-precision miR-21-targeted therapy in a single HeLa cell with sensitive photoelectrochemical (PEC) feedback. Targeting the representative oncogenic miR-21, the as-functionalized nanopipette permits direct intracellular drug administration with precisely controllable dosages, and the corresponding therapeutic effects can be sensitively transduced by a PEC sensing interface that selectively responds to the indicator level of cytosolic caspase-3. The experimental results reveal that injection of ca. 4.4 × 10 -20 mol miR-21 inhibitor, i.e., 26488 copies, can cause the obvious therapeutic action in the targeted cell. This work features a solution to obtain the accurate knowledge of how a certain miR-drug with specific dosages treats the cells and thus provides an insight into futuristic high-precision clinical miR therapy using personalized medicine, provided that the prerequisite single-cell experiments are courses of personalized customization.
Keyphrases
- single cell
- cell proliferation
- long non coding rna
- long noncoding rna
- rna seq
- high throughput
- healthcare
- quantum dots
- emergency department
- induced apoptosis
- cancer therapy
- high resolution
- cell death
- stem cells
- sensitive detection
- cell cycle arrest
- oxidative stress
- bone marrow
- genome wide
- drug delivery
- mass spectrometry
- cross sectional
- label free
- endoplasmic reticulum stress
- adverse drug
- solid phase extraction
- smoking cessation