Anticancer and apoptotic activity in neuroblastoma SK-N-SH using phospholipid extract from bone of Scomberomorus niphonius.
Anley Teferra KiddaneVikash Chandra RoyMin-Jae KangMaheshkumar Prakash PatilByung-Soo ChunGun-Do KimPublished in: Chemical biology & drug design (2022)
Among various types children's health challenges, neuroblastoma is the most serious solid neoplasm forming outside the cranium. Scomberomorus niphonius is a valuable edible fish that has been widely used for a meal. In this study, we obtained phospholipid extract from the bone of S. niphonius with the supercritical CO 2 extraction method and tested anticancer activity with a cell viability assay. The phospholipid showed anticancer activity on neuroblastoma SK-N-SH cells, and the anticancer activity was presented with an IC 50 of 710.25 ± 28.31 μg/ml, but did not show a significant toxicity on HUVEC cell lines. Western blot was used to detect signaling proteins; Bak, caspase-9, caspase-8, caspase-3, Bax, and IκBα were increased, whereas IKKβ and NFκB were downregulated in experimental groups compared to untreated groups. Gene expression was revealed by RT-qPCR, and the fold ratio of Apaf-1, cytochrome-c, caspase-9, caspase-3, and Bax genes' expression was raised in treated groups, implying apoptosis. Gel electrophoresis revealed that the experimental groups had more fragmented DNA than the control group. The study shows that a phospholipid extract from S. niphonius' bone could be used as a biological origin of anticancer activity in neuroblastoma SK-N-SH cells.
Keyphrases
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- cell death
- cell cycle arrest
- signaling pathway
- gene expression
- bone mineral density
- fatty acid
- healthcare
- pi k akt
- mental health
- public health
- young adults
- dna methylation
- south africa
- bone loss
- postmenopausal women
- low grade
- circulating tumor
- genome wide
- health information
- high throughput
- newly diagnosed
- lps induced
- body composition
- human health
- transcription factor