circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3.
Yizhen HuangZhenlei ZhangJianle WangShu-Ying ShenTeng YaoYining XuZizheng ChenBin FangJianjun MaPublished in: Experimental & molecular medicine (2021)
The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene (ATP1B3) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3. The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD.
Keyphrases
- long non coding rna
- poor prognosis
- cell proliferation
- endothelial cells
- long noncoding rna
- induced apoptosis
- induced pluripotent stem cells
- single cell
- dna damage
- healthcare
- atomic force microscopy
- mass spectrometry
- gene expression
- cell death
- cell cycle arrest
- copy number
- transcription factor
- oxidative stress
- signaling pathway
- bone marrow
- endoplasmic reticulum stress
- electronic health record