Influence of Antibiotics on Functionality and Viability of Liver Cells In Vitro.
Sandra DoßCorinne BlessingKatharina HallerGeorg RichterMartin SauerPublished in: Current issues in molecular biology (2022)
(1) Antibiotics are an important weapon in the fight against serious bacterial infections and are considered a common cause of drug-induced liver injury (DILI). The hepatotoxicity of many drugs, including antibiotics, is poorly analyzed in human in vitro models. (2) A standardized assay with a human hepatoma cell line was used to test the hepatotoxicity of various concentrations (Cmax, 5× Cmax, and 10× Cmax) of antibiotics. In an ICU, the most frequently prescribed antibiotics, ampicillin, cefepime, cefuroxime, levofloxacin, linezolid, meropenem, rifampicin, tigecycline, and vancomycin, were incubated with HepG2/C3A cells for 6 days. Cell viability (XTT assay, LDH release, and vitality), albumin synthesis, and cytochrome 1A2 activity were determined in cells. (3) In vitro, vancomycin, rifampicin, and tigecycline showed moderate hepatotoxic potential. The antibiotics ampicillin, cefepime, cefuroxime, levofloxacin, linezolid, and meropenem were associated with mild hepatotoxic reactions in test cells incubated with the testes Cmax concentration. Rifampicin and cefuroxime showed significantly negative effects on the viability of test cells. (4) Further in vitro studies and global pharmacovigilance reports should be conducted to reveal underlying mechanism of the hepatotoxic action of vancomycin, rifampicin, tigecycline, and cefuroxime, as well as the clinical relevance of these findings.
Keyphrases
- induced apoptosis
- cell cycle arrest
- mycobacterium tuberculosis
- endothelial cells
- endoplasmic reticulum stress
- cell death
- drug induced
- oxidative stress
- high throughput
- acinetobacter baumannii
- dna methylation
- gene expression
- klebsiella pneumoniae
- risk assessment
- drug resistant
- high intensity
- genome wide
- single cell
- pluripotent stem cells
- gram negative