Chromatin Profiling of Human Naïve Pluripotent Stem Cells.
Adam BendallClaudia I SemprichPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
Chromatin immunoprecipitation combined with high-throughput sequencing (ChIP-sequencing) facilitates the genome-wide mapping of DNA sequences that are enriched for specific chromatin-binding proteins or histone post-translational modifications. More recently developed chromatin profiling methods called Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and Cleavage Under Targets and Tagmentation (CUT&Tag) have adapted the ChIP-sequencing approach to produce similar data from a smaller amount of starting material, and while overcoming many of the conventional drawbacks of ChIP-sequencing. Here, we present detailed protocols for ChIP-seq, CUT&RUN, and CUT&Tag to profile genome-wide protein-DNA interactions in naïve human pluripotent stem cells.
Keyphrases
- pluripotent stem cells
- genome wide
- single cell
- dna methylation
- high throughput
- circulating tumor cells
- high throughput sequencing
- circulating tumor
- rna seq
- gene expression
- copy number
- dna binding
- dna damage
- transcription factor
- cell free
- high resolution
- oxidative stress
- machine learning
- big data
- electronic health record
- small molecule
- protein protein
- amino acid
- nucleic acid
- high density