Efficiency of bacteriological transport media quality control.
Lubov Viktorovna DomotenkoT P MorozovaA P ShepelinA Yu MironovPublished in: Klinicheskaia laboratornaia diagnostika (2021)
The aim of the study is to assess the effectiveness of quality control of transport environments in accordance with the requirements of domestic and international standards using aerobic and optional anaerobic microorganisms. The results of a comparative assessment of the survival of 10 strains of aerobic and optional anaerobic microorganisms after storage in the semi-liquid transport environment of Ames for 24 and 48 hours at temperatures (4-8)0 C and (20-25)0 C in accordance with the requirements of domestic and international standards are presented. The methodology described in ICC 4.2.2316 causes a number of technical difficulties leading to non-reproducible results. The survival rate of microorganisms in semi-liquid transport environments under ISO 11133-2016 is of high quality. The quantitative assessment is based on two Swab Elution and Roll Plate methods recommended by the CLSI M40-A2 standard.