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Synthesis of 5'-Thio-3'-O-ribonucleoside Phosphoramidites.

Nan-Sheng LiJun LuJoseph A Piccirilli
Published in: The Journal of organic chemistry (2017)
The chemical synthesis of phosphoramidite derivatives of all four 5'-deoxy-5'-thioribonucleosides is described. These phosphoramidites contained trityl (A, G, C, and U), dimethoxytrityl (A and G), or tert-butyldisulfanyl (G) as the 5'-S-protecting group. The application of several of these phosphoramidites for solid-phase synthesis of oligoribonucleotides containing a 2'-O-photocaged 5'-S-phosphorothiolate linkage or 5'-thiol-labeled RNAs is also further investigated.
Keyphrases
  • genome wide
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  • dna methylation
  • atomic force microscopy
  • high resolution
  • mass spectrometry
  • hiv infected