ATP-dependent chromatin remodeller brahma related gene 1 promotes keratinocyte migration and modulates cell Signalling during wound healing in human skin.
Carl KellettRanjit K BhogalNatalia V BotchkarevaMichael Y FessingPublished in: Experimental dermatology (2024)
Skin wound healing is driven by proliferation, migration and differentiation of several cell types that are controlled by the alterations in the gene expression programmes. Brahma Gene 1 (BRG1) (also known as SMARCA4) is a core ATPase in the BRG1 Associated Factors (BAF) ATP-dependent chromatin remodelling complexes that alter DNA-histone interaction in chromatin at the specific gene regulatory elements resulting in increase or decrease of the target gene transcription. Using siRNA mediated suppression of BRG1 during wound healing in a human ex vivo and in vitro (scratch assay) models, we demonstrated that BRG1 is essential for efficient skin wound healing by promoting epidermal keratinocytes migration, but not their proliferation or survival. BRG1 controls changes in the expression of genes associated with gene transcription, response to wounding, cell migration and cell signalling. Altogether, our data revealed that BRG1 play positive role in skin repair by promoting keratinocyte migration and impacting the genes expression programmes associated with cell migration and cellular signalling.
Keyphrases
- wound healing
- genome wide
- cell migration
- gene expression
- dna methylation
- transcription factor
- genome wide identification
- single cell
- copy number
- dna damage
- cell therapy
- poor prognosis
- endothelial cells
- signaling pathway
- genome wide analysis
- soft tissue
- machine learning
- electronic health record
- cell free
- single molecule
- deep learning
- bone marrow
- binding protein
- circulating tumor cells
- circulating tumor
- nucleic acid