The effect of osteoporotic and non-osteoporotic individuals' T cell-derived exosomes on osteoblast cells' bone remodeling related genes expression and alkaline phosphatase activity.
Mohammad Hasan OmidvarMohammad Sadegh Soltani-ZangbarMajid ZamaniRoza MotavalliMehdi JafarpoorSanam DolatiMajid AhmadiAmir MehdizadehAlireza KhabbaziMehrzad HajialiloMehdi YousefiPublished in: BMC research notes (2022)
Runx2, type I collagen, osteopontin, and osteocalcin expression decreased in osteoblasts treated by osteoporotic T cell exosomes. In contrast, an increased expression of the mentioned genes was observed following non-osteoporotic T cell exosome treatment. Additionally, osteoblast alkaline phosphatase (ALP) activity treated with non-osteoporotic T cell exosomes increased. However, this activity decreased in another group. Our data demonstrated that T cell exosomes obtained from osteoporotic and non-osteoporotic individuals could alter the osteoblastic function and gene expression by affecting the genes essential for bone remodeling.
Keyphrases
- bone mineral density
- postmenopausal women
- mesenchymal stem cells
- poor prognosis
- gene expression
- body composition
- stem cells
- induced apoptosis
- genome wide
- binding protein
- magnetic resonance
- magnetic resonance imaging
- transcription factor
- bone regeneration
- computed tomography
- bone marrow
- machine learning
- oxidative stress
- soft tissue
- genome wide identification
- bioinformatics analysis
- bone loss