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De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea).

Takuma SakamotoShunya SasakiNobuki YamaguchiMiho NakanoHiroki SatoKikuo IwabuchiHiroko TabunokiRichard J SimpsonHidemasa Bono
Published in: BMC research notes (2021)
Here, we report RNA sequencing of midgut transcriptome of Entoria okinawaensis, a prominent Japanese flightless-wingless stick insect, and the comparative analysis of its transcriptome in publicly available midgut transcriptomes obtained from seven stick insect species. A gene enrichment analysis for differentially expressed genes, including those obtained from winged vs wingless and flight vs flightless genes comparisons, revealed that carbohydrate metabolic process-related genes were highly expressed in the winged stick insect group. We also found that the expression of the mitochondrial enolase superfamily member 1 transcript was significantly higher in the winged stick insect group than in the wingless stick insect group. Our findings could indicate that carbohydrate metabolic processes are related to the evolutionary process through which stick insects gain the ability of flight.
Keyphrases
  • genome wide
  • aedes aegypti
  • single cell
  • rna seq
  • genome wide identification
  • dna methylation
  • gene expression
  • poor prognosis
  • zika virus
  • oxidative stress
  • copy number
  • physical activity
  • genetic diversity