T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device.
Yang LiuXiaoou RenJiandong WuJohn A WilkinsFrancis LinPublished in: Micromachines (2022)
Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for subsequent in situ fixation and direct fluorescence analysis of the cells in the microchannel.
Keyphrases
- single cell
- high throughput
- cell migration
- rna seq
- study protocol
- induced apoptosis
- randomized controlled trial
- circulating tumor cells
- cell cycle arrest
- clinical trial
- stem cells
- minimally invasive
- high resolution
- escherichia coli
- cancer therapy
- endoplasmic reticulum stress
- biofilm formation
- risk assessment
- case control
- peripheral blood
- mesenchymal stem cells
- drug delivery
- bone marrow
- pseudomonas aeruginosa
- human health
- climate change
- label free
- double blind