Protective effects of Lactobacillus reuteri SJ-47 strain exopolysaccharides on human skin fibroblasts damaged by UVA radiation.
Jingsha ZhaoHao FuYongtao ZhangMeng LiDongdong WangDan ZhaoJiachan ZhangChangtao WangPublished in: Bioresources and bioprocessing (2022)
Ultraviolet rays in sunlight can cause skin damage and premature aging. This study demonstrates that Lactobacillus reuteri SJ-47 strain exopolysaccharides (EPS) protect human skin fibroblasts (HSF) under UVA radiation. During the course of the experiments, we investigate the oxidative stress protection and antiaging effects of exopolysaccharides on HSF at the biochemical, cellular, and molecular levels. The results show that EPS can increase the antioxidant capacity of cells, decrease the amount of reactive-oxygen species (ROS) and malondialdehyde (MDA), while improve the expression of antioxidant enzymes. At the same time, EPS can increase collagen content, which can effectively regulate the expression of genes in the senescence and apoptosis pathways, and delay skin photoaging caused by UVA irradiation.
Keyphrases
- oxidative stress
- cell cycle arrest
- induced apoptosis
- reactive oxygen species
- dna damage
- cell death
- poor prognosis
- heat shock
- wound healing
- endoplasmic reticulum stress
- diabetic rats
- ischemia reperfusion injury
- soft tissue
- pi k akt
- extracellular matrix
- radiation induced
- disease activity
- binding protein
- endothelial cells
- radiation therapy
- gene expression
- long non coding rna
- anti inflammatory
- systemic lupus erythematosus
- breast cancer cells
- transcription factor
- genome wide identification
- single molecule