Histone lactylation promotes malignant progression by facilitating USP39 expression to target PI3K/AKT/HIF-1α signal pathway in endometrial carcinoma.
Sitian WeiJun ZhangRong ZhaoRui ShiLanfen AnZhicheng YuQi ZhangJiarui ZhangYuwei YaoHaojia LiHongbo WangPublished in: Cell death discovery (2024)
Histone lactylation has been reported to involve in tumorigenesis and development. However, its biological regulatory mechanism in endometrial carcinoma (EC) is yet to be reported in detail. In the present study, we evaluated the modification levels of global lactylation in EC tissues by immunohistochemistry and western blot, and it was elevated. The non-metabolizable glucose analog 2-deoxy-d-glucose (2-DG) and oxamate treatment could decrease the level of lactylation so as to inhibit the proliferation and migration ability, induce apoptosis significantly, and arrest the cell cycle of EC cells. Mechanically, histone lactylation stimulated USP39 expression to promote tumor progression. Moreover, USP39 activated PI3K/AKT/HIF-1α signaling pathway via interacting with and stabilizing PGK1 to stimulate glycolysis. The results of present study suggest that histone lactylation plays an important role in the progression of EC by promoting the malignant biological behavior of EC cells, thus providing insights into potential therapeutic strategies for endometrial cancer.
Keyphrases
- cell cycle arrest
- pi k akt
- signaling pathway
- cell cycle
- cell proliferation
- endometrial cancer
- induced apoptosis
- cell death
- poor prognosis
- dna methylation
- oxidative stress
- endothelial cells
- endoplasmic reticulum stress
- epithelial mesenchymal transition
- long non coding rna
- blood pressure
- type diabetes
- metabolic syndrome
- transcription factor
- climate change
- skeletal muscle
- weight loss
- risk assessment