Reduced Shmt2 Expression Impairs Mitochondrial Folate Accumulation and Respiration, and Leads to Uracil Accumulation in Mouse Mitochondrial DNA.

Joanna L FiddlerYuwen XiuJamie E BlumSimon G LamarreWhitney N PhinneySally P StablerMargaret E BrosnanJohn T BrosnanAnna E Thalacker-MercerMartha S Field

Published in: The Journal of nutrition (2022)

This study demonstrates that Shmt2 heterozygosity and dietary folate deficiency impair mitochondrial dTMP synthesis in mice, as evidenced by the increased uracil in mtDNA. In addition, Shmt2 heterozygosity impairs mitochondrial function in MEF cells. These findings suggest that elevated uracil in mtDNA may impair mitochondrial function.

Keyphrases

mitochondrial dna
copy number
oxidative stress
induced apoptosis
poor prognosis
cell cycle arrest
genome wide
signaling pathway
type diabetes
high fat diet induced
metabolic syndrome
endoplasmic reticulum stress
cell proliferation