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Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins.

Toshihisa NomuraKomaki InoueYukiko Uehara-YamaguchiKoji YamadaOsamu IwataKengo SuzukiKeiichi Mochdia
Published in: Plant biotechnology journal (2019)
Keyphrases
  • highly efficient
  • crispr cas
  • genome editing
  • heavy metals
  • wastewater treatment
  • cancer therapy
  • binding protein
  • nucleic acid