Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins.
Toshihisa NomuraKomaki InoueYukiko Uehara-YamaguchiKoji YamadaOsamu IwataKengo SuzukiKeiichi MochdiaPublished in: Plant biotechnology journal (2019)