CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H.
Hansol KimSeoyoung LeeJinhwan LeeHyun Gyu ParkPublished in: Chemical communications (Cambridge, England) (2022)
We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL -1 , which is quite superior to those of alternative approaches.