Therapeutic potential of topically administered γ-AlOOH on 2,4-dinitrochlorobenzene-induced atopic dermatitis-like lesions in Balb/c mice.
Dong-Ho BakEsther LeeByung Chul LeeMi Ji ChoiTae-Rin KwonJi-Yeon HongSeog-Kyun MunKeugrae LeeSungyup KimJungtae NaBeom Joon KimPublished in: Experimental dermatology (2019)
Boehmite (γ-AlOOH) has a wide range of applications in a variety of industrial and biological fields. However, little is known about its potential roles in skin diseases. The current study investigated its effect on atopic dermatitis (AD). Following characterization, cytotoxicity, pro-inflammatory response and oxidative stress associated with boehmite were assessed, using TNF-α-induced keratinocytes and mast cells. In addition, therapeutic effects of boehmite, topically administered to Balb/c mice induced by 2,4-dinitrochlorobenzene (DNCB), were evaluated. Expression of cytokines (TLSP, IL-25 and IL-33) and the generation of ROS from keratinocytes induced by TNF-α were significantly inhibited by boehmite without affecting cell viability. MAPKs (ERK, JNK and p38) required for cytokine expression were suppressed by boehmite treatment. Up-regulation of cytokines (TSLP, IL-4, IL-5, IL-13, RANTES) in human mast cells treated with phorbol 12-myristate 13-acetate and calcium ionophore was also suppressed by boehmite. Boehmite improved the AD severity score, epidermal hyperplasia and transepidermal water loss in DNCB-induced AD-like lesions. Moreover, Th2-mediated cytokine expression, mast cell hyperplasia and destruction of the skin barrier were improved by boehmite treatment. Overall, we demonstrated that boehmite may potentially protect against AD.
Keyphrases
- atopic dermatitis
- diabetic rats
- poor prognosis
- high glucose
- oxidative stress
- inflammatory response
- wound healing
- endothelial cells
- rheumatoid arthritis
- signaling pathway
- cell death
- dna damage
- binding protein
- drug induced
- cell proliferation
- type diabetes
- induced apoptosis
- long non coding rna
- reactive oxygen species
- high resolution
- soft tissue
- skeletal muscle
- metabolic syndrome
- combination therapy
- atomic force microscopy
- anti inflammatory
- lipopolysaccharide induced
- lps induced