Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer.
Marina Dudea-SimonDan MihuLaura-Ancuta PopRazvan CiorteaAndrei Mihai MalutanDoru DiculescuCristina Alexandra CiocanRoxana Maria CojocneanuVasile SimonCarmen BucuriRadu Mocan-HognogiCornelia BraicuIoana Berindan NeagoePublished in: International journal of molecular sciences (2022)
Background : Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. Methods : We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. Results : We observed higher expression levels for MEG3, DAPK1 , MLH1 and MALAT1 in CIN samples than in normal samples, whereas TIMP3 and SOX1 had lower expression levels. For cancer samples, DAPK1 , MLH1 and MALAT1 had higher expression, and MEG3 , TIMP3 and SOX1 had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only MEG3 gene showed a statistically significant difference. The expression of miR-205-5p was lower in both CIN and cancer samples compared to normal samples. Conclusion: Decreased MEG3 expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of TIMP3 , SOX1 , MLH1 , MALAT1 and miR-205-5p could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.
Keyphrases
- poor prognosis
- high grade
- stem cells
- papillary thyroid
- binding protein
- squamous cell carcinoma
- emergency department
- type diabetes
- coronary artery disease
- prostate cancer
- long non coding rna
- rectal cancer
- pregnant women
- cardiovascular disease
- cardiovascular events
- polycystic ovary syndrome
- metabolic syndrome
- quantum dots
- young adults
- skeletal muscle
- dna methylation
- lymph node metastasis
- radical prostatectomy
- drug induced
- breast cancer risk