Red-emissive Dual-state Fluorogenic Probe for Wash-free Imaging of Lipid Droplets in Living Cells and Fatty Liver Tissues.
Hui WangCuifeng ZhangLei HuFang TangYaxuan WangFeiyang DingJingwen LuAixiang DingPublished in: Chemistry, an Asian journal (2023)
Lipid droplet (LD) dysfunction can result in various diseases, such as nonalcoholic fatty liver disease. Imaging agents built on dual-state emission (DSE) molecules that fluoresce in both dilute solutions and the aggregated state are receiving attention as this type of probe could provide bright fluorescence signals at variable concentrations, avoiding false signal readout caused by the concentration fluctuation in living systems. Herein, we identified a red emissive molecule featuring DSE, from three newly synthesized molecules, for specific detection of LDs in live cells. The bioimaging abilities have been well confirmed by optical spectroscopies, theoretical calculations, cell experiments, as well as animal studies. The DSE probe is effective for LD detection at concentrations ranging from 1 μM to 100 μM while retaining high brightness and signal fidelity. This study provides a knowledge base for the future design of DSE-active fluorescent probes for understanding LD-related diseases.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- high resolution
- single cell
- induced apoptosis
- fatty acid
- loop mediated isothermal amplification
- label free
- healthcare
- real time pcr
- oxidative stress
- gene expression
- cell cycle arrest
- molecular dynamics simulations
- quantum dots
- cell therapy
- current status
- molecular dynamics
- endoplasmic reticulum stress
- high speed
- case control
- pi k akt