Ultra-high-throughput mass spectrometry in drug discovery: fundamentals and recent advances.
Jon D WilliamsFan PuJames W SawickiNathaniel L ElsenPublished in: Expert opinion on drug discovery (2023)
The fast analysis time provided by uHT-MS is transforming how biochemical and chemical assays are performed in drug discovery. The potential to associate phenotypic responses produced by 1000's of compound treatments with changes in endogenous metabolite and lipid signals is becoming feasible. With the augmentation of simple, fast, high-throughput sample preparation, the scope of uHT-MS usage will increase. However, it likely will not supplant LC-MS for analyses that require low detection limits from complex matrices or characterization of complex biotherapeutics such as antibody-drug conjugates.
Keyphrases
- drug discovery
- high throughput
- mass spectrometry
- liquid chromatography
- high resolution
- single cell
- multiple sclerosis
- ms ms
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- cancer therapy
- label free
- loop mediated isothermal amplification
- soft tissue
- fatty acid
- climate change
- simultaneous determination