Communication-Microelectrode Detection of Cholesterol Efflux from the Human Buccel Mucosa.
Xiaochun YuThomas J KelleyHillel J ChielJames D BurgessPublished in: Journal of the Electrochemical Society (2016)
It has previously demonstrated that cholesterol efflux from the cell plasma membrane is increased in a mouse model of cystic fibrosis (CF) compared to a wild-type control. A noninvasive means of characterizing plasma membrane cholesterol efflux at the surface of airway tissue of CF patients is needed to extend the trends found in animal models of CF to the human disease state. Microelectrode-induced cholesterol efflux from the plasma membrane of cells at the surface of tissue is proposed as a strategy to demonstrate increased cholesterol efflux for CF in human subjects. Data demonstrating detection of cholesterol efflux from the human buccal mucosa is reported as proof-of-concept for an in vivo diagnostic assay.
Keyphrases
- cystic fibrosis
- endothelial cells
- low density lipoprotein
- induced pluripotent stem cells
- mouse model
- pluripotent stem cells
- pseudomonas aeruginosa
- high glucose
- wild type
- deep brain stimulation
- high throughput
- deep learning
- mesenchymal stem cells
- oxidative stress
- prognostic factors
- cell cycle arrest
- artificial intelligence
- real time pcr
- pi k akt
- data analysis
- stress induced